ISO 4133:1979

Meat and meat products — Determination of glucono-delta-lactone content (Reference method)

Publication date:   Feb 1, 1979

95.99   Withdrawal of Standard   Oct 2, 2002

General information

95.99   Withdrawal of Standard   Oct 2, 2002

ISO

ISO/TC 34/SC 6 Meat, poultry, fish, eggs and their products

International Standard

67.120.10   Meat and meat products

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Scope

The method consists in extracting of the compound with ice-cold perchloric acid solution, centrifuging, decanting and filtrating, followed by hydrolysis of the compound in a portion of the filtrate with potassium hydroxide into gluconate. Transformation of the gluconate in the extract by the following reactions: 1) with adenosine-5-triphosphate (ATP) and 2) with concomitant reduction of an equivalent amount of nicotinamide adenine dinucleotide phosphate (NADP). Photometric measurement of the amount of nicotinamide adenine dinucleotide phosphate (reduced) (NADPH) formed.

Life cycle

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WITHDRAWN
ISO 4133:1979
95.99 Withdrawal of Standard
Oct 2, 2002