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ISO 18862:2025

Coffee and coffee products — Determination of acrylamide — Methods using high-performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) and gas chromatography with mass spectrometric detection (GC-MS) after derivatization

Publication date:   Nov 27, 2025

General information

60.60   Standard published   Nov 27, 2025

ISO

ISO/TC 34/SC 15 Coffee

International Standard

67.140.20   Coffee and coffee substitutes

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Scope

This document specifies methods for the determination of acrylamide in coffee and coffee products by extraction with water, clean-up by solid-phase extraction (SPE) and determination by high-performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) and gas chromatography with mass spectrometric detection (GC-MS) after derivatization. The methods were validated in a validation study for roasted coffee, soluble coffee, coffee substitutes and coffee products with ranges from 53 μg/kg to 612,1 μg/kg.

Life cycle

PREVIOUSLY

WITHDRAWN
ISO 18862:2016

NOW

PUBLISHED
ISO 18862:2025
60.60 Standard published
Nov 27, 2025