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EN ISO 12966-2:2011

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids (ISO 12966-2:2011) EN ISO 12966-2:2011

Publication date:   Apr 19, 2011

General information

99.60 Withdrawal effective   Apr 26, 2017

CEN

CEN/TC 307 Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis

European Norm

67.200.10   Animal and vegetable fats and oils

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Scope

ISO 12966-2:2011 specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements four methylation methods are specified, namely: a) a "rapid" transmethylation procedure under alkaline conditions; b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions; c) a BF3 transmethylation procedure; d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GC), thin-layer chromatography (TLC), and infrared spectrometry (IR).

Life cycle

PREVIOUSLY

WITHDRAWN
EN ISO 5509:2000

NOW

WITHDRAWN
EN ISO 12966-2:2011
99.60 Withdrawal effective
Apr 26, 2017

REVISED BY

PUBLISHED
EN ISO 12966-2:2017