ISO 4962:2024

Nanotechnologies — In vitro acute nanoparticle phototoxicity assay

Publication date:   Nov 5, 2024

General information

60.60 Standard published   Nov 5, 2024

ISO

ISO/TC 229 Nanotechnologies

International Standard

07.120   Nanotechnologies

Buying

  Published

PDF - €156.09

  English  



Buy

Scope

This document specifies a procedure to evaluate acute phototoxicity of nanoparticles (NPs), suspended in cell culture media, by measuring the relative reduction in cellular viability under near ultraviolet (UVA) exposure (315 nm to 400 nm).
The measurement is intended to assess the potential for acute phototoxicity of NPs by comparing NP photoactivity in vitro to a positive control chlorpromazine. It is not designed to predict other joint effects of nanomaterials and light, such as genotoxicity, photo-allergy or photo-mutagenicity. The method is also applicable to NP aggregates and agglomerates.

Life cycle

NOW

PUBLISHED
ISO 4962:2024
60.60 Standard published
Nov 5, 2024