20.00 New project registered in TC/SC work programme Apr 4, 2024
ISO
ISO/TC 34/SC 16 Horizontal methods for molecular biomarker analysis
Technical Specification
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of chickpea-specific DNA derived from food. PCR amplification reactions in which sufficient amounts of DNA are extracted from the relevant substrate can be used to detect the genetic material of chickpeas. The target sequence is a partial fragment of the chickpea chloroplast import apparatus 2-like gene in chromosome Ca7 (GenBank accession number CP039337.1). The provided PCR assay for this target has an absolute detection limit of 5 copies per reaction, with ≥ 95% reproducibility at this concentration (LOD95%).
IN_DEVELOPMENT
ISO/AWI TS 24910-1
20.00
New project registered in TC/SC work programme
Apr 4, 2024