ISO 23036-2:2021

Microbiology of the food chain — Methods for the detection of Anisakidae L3 larvae in fish and fishery products — Part 2: Artificial digestion method ISO 23036-2:2021

Publication date:   Apr 21, 2021

General information

60.60 Standard published   Apr 21, 2021


ISO/TC 34/SC 9 Microbiology

International Standard

07.100.30   Food microbiology



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This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme.
The artificial digestion method[4][5][6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present.
This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.

Life cycle


ISO 23036-2:2021
60.60 Standard published
Apr 21, 2021