ISO 20397-3:2025

Biotechnology — Massively parallel sequencing — Part 3: General requirements and guidance for metagenomics

Publication date:   Jul 23, 2025

General information

90.92   Standard to be revised   Jun 17, 2026

ISO

ISO/TC 276/SC 1

International Standard

07.080   Biology. Botany. Zoology

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Scope

This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, and generating and analysing metagenomics sequence data obtained from massive parallel sequencing platforms. The specified metagenomics process includes the following stages:
a)       sampling strategy and process, including type, storage, transportation, extraction, quality;
b)       nucleic acid library preparation
c)        design and review process including sequencing strategy and assessment;
d)       database construction;
e)       bioinformatics analysis and report
f)         validation and verification for bioinformatics pipeline, and database
This document applies to laboratories and research organizations.

Life cycle

NOW

PUBLISHED
ISO 20397-3:2025
90.92 Standard to be revised
Jun 17, 2026

REVISED BY

IN_DEVELOPMENT
ISO/AWI 20397-3