ISO/AWI 20397-3

Biotechnology — NGS — Part 3: General requirements and guidance for metagenomics ISO/AWI 20397-3

Scope

This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, and generating and analysing metagenomics sequence data obtained from massive parallel sequencing platforms. The specified metagenomics process includes the following stages:
a)       sampling strategy and process, including type, storage, transportation, extraction, quality;
b)       nucleic acid library preparation
c)        design and review process including sequencing strategy and assessment;
d)       database construction;
e)       bioinformatics analysis and report
f)         validation and verification for bioinformatics pipeline, and database
This document applies to laboratories and research organizations.