ISO 14674:2005

Milk and milk powder — Determination of aflatoxin M1 content — Clean-up by immunoaffinity chromatography and determination by thin-layer chromatography ISO 14674:2005

Publication date:   May 27, 2005

Scope

ISO 14674|IDF 190:2005 specifies a method for the determination of the aflatoxin M1 (AFM1) content of milk and milk powder by a method including a clean-up step using immunoaffinity chromatography followed by a thin-layer chromatography (IAC-TLC).
The method is applicable to raw milk, low fat or skimmed liquid milk and milk powder.
The lowest quantity of AFM1 that can commonly be determined is 2 ng, which corresponds to a limit of quantification close to 0,10 micrograms per litre for liquid milk or dissolved milk powder (for a spot of 20 microlitres).