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ISO 12966-3:2016

Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)

Publication date:   May 12, 2016

General information

90.93 Standard confirmed   Dec 8, 2021

ISO

ISO/TC 34/SC 11 Animal and vegetable fats and oils

International Standard

67.200.10   Animal and vegetable fats and oils

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Scope

ISO 12966-3:2016 specifies a rapid base-catalysed transesterification method for fats and oils with trimethylsulfonium hydroxide (TMSH) to prepare fatty acid methyl esters. The method is exclusively applicable to the preparation of methyl esters of fats and oils for gas liquid chromatographic (GLC) analysis. It is applicable to all fats and oils, but excluding those coming from milk and milk products. Isomerization of unsaturated fatty acids only occurs to a minor extent and isomerized fatty acids are only present at the determination limit. As isomerization takes place, the procedure is not recommended for conjugated linoleic acid (CLA).
Only about 70 % to 80 % of the free fatty acids are esterified. In the case of conjugated cyclopropyl and cyclopropenyl fatty acids, side reactions may occur, but these do not interfere with the determination of the fatty acids.

Life cycle

PREVIOUSLY

WITHDRAWN
ISO 12966-3:2009

NOW

PUBLISHED
ISO 12966-3:2016
90.93 Standard confirmed
Dec 8, 2021