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ISO 12966-2:2017

Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 2: Preparation of methyl esters of fatty acids

Publication date:   Mar 31, 2017

General information

90.93 Standard confirmed   Nov 8, 2022

ISO

ISO/TC 34/SC 11 Animal and vegetable fats and oils

International Standard

67.200.10   Animal and vegetable fats and oils

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Scope

ISO 12966-2:2017 specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely:
a) a "rapid" transmethylation procedure under alkaline conditions;
b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions;
c) a BF3 transmethylation procedure;
d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

Life cycle

PREVIOUSLY

WITHDRAWN
ISO 12966-2:2011

NOW

PUBLISHED
ISO 12966-2:2017
90.93 Standard confirmed
Nov 8, 2022