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ISO 12966-2:2011

Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 2: Preparation of methyl esters of fatty acids

Publication date:   Feb 4, 2011

95.99 Withdrawal of Standard   Mar 31, 2017

General information

95.99 Withdrawal of Standard   Mar 31, 2017

ISO

ISO/TC 34/SC 11 Animal and vegetable fats and oils

International Standard

67.200.10   Animal and vegetable fats and oils

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Scope

ISO 12966-2:2011 specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements four methylation methods are specified, namely: a) a "rapid" transmethylation procedure under alkaline conditions; b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions; c) a BF3 transmethylation procedure; d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GC), thin-layer chromatography (TLC), and infrared spectrometry (IR).

Life cycle

PREVIOUSLY

WITHDRAWN
ISO 5509:2000

NOW

WITHDRAWN
ISO 12966-2:2011
95.99 Withdrawal of Standard
Mar 31, 2017

REVISED BY

PUBLISHED
ISO 12966-2:2017