FprEN 17899

Water quality - Spectrophotometric determination of chlorophyll-a content by ethanol extraction for the routine monitoring of water quality

Publication date:   Sep 22, 2022

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50.60 Close of voting. Proof returned by secretariat   May 9, 2024

CEN

CEN/TC 230 Water analysis

European Norm

13.060.70   Examination of biological properties of water | 71.040.50   Physicochemical methods of analysis

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Scope

This document describes a spectrophotometric method for determining the chlorophyll-a content corrected for phaeopigments as a measure of the amount of phytoplankton for all types of surface water including marine water. The determination limit is usually 2 µg/l to 5 µg/l and is calculated by each laboratory individually. It can be as low as 0,5 µg/l using 2 l of sample (or even more) and a 50 mm cuvette.
NOTE In some measurement programs like marine studies on time series data and ecological status/classification no correction for phaeopigments is used and acidification is omitted, e.g. as recommended by OSPAR.

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FprEN 17899
50.60 Close of voting. Proof returned by secretariat
May 9, 2024