EN ISO 29981:2026

Milk products - Enumeration of bifidobacteria - Colony-count technique (ISO 29981:2024) EN ISO 29981:2026

Publication date:   May 18, 2026

General information

60.60   Standard published   Mar 18, 2026

CEN

CEN/TC 302 Milk and milk products - Methods of sampling and analysis

European Norm

67.100.01   Milk and milk products in general

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Scope

This document specifies a method for the selective enumeration of bifidobacteria in milk products by using a colony-count technique at 37 °C under anaerobic conditions.
The method is applicable to milk products, such as fermented (e.g. yoghurts) and non-fermented milks (e.g. pasteurized milks, skim milks, whey protein concentrates), milk powders and formulae (e.g. infant formulae, follow-up formulae for older infants, products for young children) where these microorganisms are present and viable, in combination with other lactic acid bacteria or alone. The method is also applicable to starter and probiotic cultures. For proposed quality criteria of dairy products, see, for example, CXS 243-2003.

Bifidobacteria used in milk products usually belong to the following species (e.g. References [7] and [10]):
— Bifidobacterium adolescentis;
— B. animalis subsp. animalis;
— B. animalis subsp. lactis;
— B. bifidum;
— B. breve;
— B. longum subsp. infantis;
— B. longum subsp. longum.

Life cycle

NOW

PUBLISHED
EN ISO 29981:2026
60.60 Standard published
Mar 18, 2026

Relations

Adopted from ISO 29981:2024 IDENTICAL




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