EN 14122:2014

Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography EN 14122:2014

Publication date:   Aug 19, 2014

General information

90.93 Standard confirmed   Oct 28, 2020

CEN

CEN/TC 275 Food analysis - Horizontal methods

European Norm

67.050   General methods of tests and analysis for food products

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This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/spray dried milk, freeze-dried mixed vegetables and freeze-dried pig's liver ranging from 0,295 mg/100 g to 0,807 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/100 g to 486 mg/100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives.
For further information on the validation, see Clause 8 and Annex B.

Life cycle

PREVIOUSLY

WITHDRAWN
EN 14122:2003

WITHDRAWN
EN 14122:2003/AC:2005

NOW

PUBLISHED
EN 14122:2014
90.93 Standard confirmed
Oct 28, 2020