ISO/WD 25017-1

Water quality — Recovery and detection methods for Acinetobacter baumannii in aquatic ecosystems — Part 1: Natural water ISO/WD 25017-1

Scope

This proposal to develop a standard method for the enhanced recovery, isolation, detection and enumeration of Acinetobacter spp. in environmental aquatic niches including agricultural, wastewater effluents, recreational, ground- and well waters. The standardized method is specific for each water type in a specified sample volume e.g., 500 mL of clean water (drinking, recreational, ground- and well water) and 0.1 to 100 mL turbid (agricultural and wastewater effluent). The sample will be membrane filtered (0.45 μm). The filter will be placed on Acinetobacter selective growth media and incubated under aerobic and microaerophilic conditions at 30 and 37°C for 18- 24 hours. An inter-laboratory ring-trial will be conducted where each participating country (minimum five) laboratories will simulate a known number of A. baumannii reference strain cells in various environmental water sources. In this stage of ring-trial, raw drinking and post-treated wastewater will be used for the assessment of optimal temperature incubation conditions for enhanced recovery from each substrate. The results will then be analyzed and based on the statistical findings, the optimum sampling and growth conditions will be described for each water type.