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ISO/TS 22939:2019

Soil quality — Measurement of enzyme activity patterns in soil samples using fluorogenic substrates in micro-well plates

Publication date:   Aug 8, 2019

General information

90.20   Standard under periodical review   Jul 15, 2026

ISO

ISO/TC 190/SC 4 Biological characterization

Technical Specification

13.080.30   Biological properties of soils

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Scope

This document specifies a method for the measurement of several enzyme activities (arylsulfatase, α −glucosidase, β -glucosidase, Cellubisidase, β -Xylosidase, phosphodiesterase (PDE), chitinase, phosphomonoesterase (PME), leucine-aminopeptidase, Alanine-aminopeptidase) simultaneously (or not) using fluorigenic substrates in soil samples. Enzyme activities of soil vary seasonally and depend on the chemical, physical and biological characteristics of soil. Its application for the detection of harmful effects of toxic chemicals or other anthropogenic impacts depends on the simultaneous comparison of enzyme activities in a control soil similar to the test soil, or on exposure tests with chemicals or treatments.

Life cycle

PREVIOUSLY

WITHDRAWN
ISO/TS 22939:2010

NOW

PUBLISHED
ISO/TS 22939:2019
90.20 Standard under periodical review
Jul 15, 2026