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ISO 21115:2019

Water quality — Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)

Publication date:   Apr 1, 2019

General information

90.93 Standard confirmed   Nov 7, 2024

ISO

ISO/TC 147/SC 5 Biological methods

International Standard

13.060.70   Examination of biological properties of water

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Scope

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).

Life cycle

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PUBLISHED
ISO 21115:2019
90.93 Standard confirmed
Nov 7, 2024