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EN ISO 17601:2018

Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil (ISO 17601:2016) EN ISO 17601:2018

Publication date:   Apr 16, 2018

General information

60.60 Standard published   Feb 21, 2018

CEN

CEN/TC 444 Environmental characterization of solid matrices

European Norm

13.080.30   Biological properties of soils

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Scope

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.
It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

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PUBLISHED
EN ISO 17601:2018
60.60 Standard published
Feb 21, 2018

REVISED BY

IN_DEVELOPMENT
prEN 17601 rev

Relations

Adopted from ISO 17601:2016 IDENTICAL