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60.60 Standard published Apr 12, 2023
CEN
CEN/TC 327 Animal feeding stuffs - Methods of sampling and analysis
European Norm
65.120 Animal feeding stuffs
Published
This document specifies a method for the determination of individual intact glucosinolates in feed materials including oilseeds and oilseed products and in compound feeds by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS).
The method specified in this document has been successfully validated by collaborative trial in the following matrices: rape seed, camelina seed, Brassica oleracea seeds, mixed oilseeds, rape seed flakes, compound feed for bovine, porcine and poultry.
The method is applicable for the quantitative determination of epiprogroitrin, glucoalyssin, glucoarabin, glucobrassicanapin, glucobrassicin, glucocamelinin, glucoerucin, glucoiberin, gluconapin, gluconapoleiferin, gluconasturtiin, glucoraphanin, glucoraphenin, glucotropaeolin, homoglucocamelinin, 4-hydroxyglucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin, progoitrin, sinalbin and sinigrin.
The concentration ranges tested in the collaborative trial for each individual glucosinolate and for the total glucosinolate content are summarized in Table 1.
[table not represented]
Legislation related to this standard
Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
PUBLISHED
EN 17853:2023
60.60
Standard published
Apr 12, 2023