EN 17298:2019

Animal feeding stuffs - Methods of sampling and analysis - Determination of benzoic and sorbic acid by High Performance Liquid Chromatography (HPLC)

Publication date:   Nov 14, 2019

General information

90.20 Standard under periodical review   Oct 15, 2024

CEN

CEN/TC 327 Animal feeding stuffs - Methods of sampling and analysis

European Norm

65.120   Animal feeding stuffs | 71.040.40   Chemical analysis

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Scope

This document specifies a method for the determination of benzoic acid and sorbic acid in animal feeding stuffs by high-performance liquid chromatography method with ultraviolet detection (HPLC-UV).
The method is intended to be used for the determination of benzoic acid and sorbic acid as active substances in feed additives, premixtures, feed materials and compound feed and for benzoic acid in water. This method determines the total extractable concentration of these organic acids and their salts.
It is advisable that the working range of the method is determined for each organic acid by the user of this standard. The lower limit of the working range depends on the matrix and the interferences encountered. It is advisable that a working range between 5 mg/l and 100 mg/l is accessible.
The method was successfully tested in an inter-laboratory study in concentrations between 0,02 % up to 9,0 %.
On the basis of the referred working range, sample weight and extraction volume, limits of quantification (LOQ), as calculated (Table 1) on the basis of a wavelength of 230 nm, can be achievable.

Related legislation

Legislation related to this standard

882/2004

Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules

Life cycle

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PUBLISHED
EN 17298:2019
90.20 Standard under periodical review
Oct 15, 2024