EN 12393-2:2008

Foods of plant origin - Multiresidue methods for the gas chromatographic determination of pesticide residues - Part 2: Methods for extraction and cleanup

Publication date:   Jul 10, 2009

Scope

This European Standard specifies methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues.
Different solvents can be used for this purpose. These pesticide residues are generally associated with other co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed, several techniques can be used.
This standard contains the following extraction and clean-up methods that have been subjected to interlaboratory studies and /or are adopted throughout Europe:
- method L: Extraction with acetone, liquid-liquid partition with dichloromethane and clean-up on a silica gel/charcoal column [1];
- method M: Extraction with acetone and liquid-liquid partition with dichloromethane/light petroleum, if necessary clean-up on Florisil® 1) [2], [3], [4];
- method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane/ethyl acetate and clean-up with gel permeation and silica gel chromatography [5], [6];
- method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography [7].
This European Standard specifies the details of methods L to P for the extraction and the clean-up of food samples of plant origin. Several solvents at different volumes are used for extraction. Techniques of clean-up are listed such as liquid-liquid partition, liquid chromatography on various adsorbents and gel permeation chromatography.
A table providing the couples (matrix/pesticide) which have been submitted to collaborative studies and a list of indicative applicability of the method to different pesticides are given for each method, wherever possible.