CEN/TS 17781:2022

Organic, organo-mineral and inorganic fertilizers - Detection of Escherichia coli CEN/TS 17781:2022

Publication date:   Jul 14, 2022

General information

60.60 Standard published   Apr 6, 2022

CEN

CEN/TC 260 Fertilizers and liming materials

Technical Specification

65.080   Fertilizers

Buying

Published

Language in which you want to receive the document.

Scope

This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009 [1]. However, the present method was not validated for blends.
This document specifies a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for the detection of the enzyme β-glucuronidase. The method is based on ISO 16649-2 [4].
Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are β-glucuronidase negative, such as Escherichia coli O157, will not be detected. Detected microorganisms are presumptively determined β-glucuronidase-positive Escherichia coli, since some Enterobacteriaceae, in particular Shigella and Salmonella, can also show β-glucuronidase activity at 44 °C.

Related legislation

Legislation related to this standard

2019/1009

Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down rules on the making available on the market of EU fertilising products and amending Regulations (EC) No 1069/2009 and (EC) No 1107/2009 and repealing Regulation (EC) No 2003/2003 (Text with EEA relevance)

Life cycle

NOW

PUBLISHED
CEN/TS 17781:2022
60.60 Standard published
Apr 6, 2022