cen:proj:28164

Detection and enumeration of Clostridium perfringens in sludges – Part 2: Macromethod (Most Probable Number) by inoculation into selective liquid medium cen:proj:28164

Scope

This draft standard method specifies a most probable number method (MPN) for the analysis, by culture of Clostridium perfringens in selective broth, in sewage sludge, samples. The user should prior to analysis validate the method for the particular type of sample they wish to analyse: sludges. This method is of particular use if determination of treatment efficiency is required as outlined in the revision
of Directive 86/278/EEC (3rd draft, CEN/TC 308-doc 525).
The method has a limit of detection of approximately 10 cfu/g wet weight.