cen:proj:28163

Detection and enumeration of Clostridium perfringens in sludges – Part 1: membrane filtration onto selective agars cen:proj:28163

Scope

This draft standard method specifies a membrane filtration procedure for the analysis, by culture of individual colonies of Clostridium perfringens on selective agar, in sewage sludge, samples. The user should prior to analysis validate the method for the particular type of sample they wish to analyse: sludges. This method is of particular use if determination of treatment efficiency is required as outlined in the
revision of Directive 86/278/EEC (3rd draft, CEN/TC 308-doc 525).
The method has a limit of detection of approximately 10 cfu/g wet weight, dependent of the solids content which at high concentrations (>20% (w/v)) can block the filtration of the sample volume through the membrane if not dilution prior to filtration.