ISO/TS 18220:2016

Water quality — Larval development test with the harpacticoid copepod Nitocra spinipes

Publication date:   Jun 16, 2016

General information

90.93 Standard confirmed   Sep 16, 2019


ISO/TC 147/SC 5 Biological methods

Technical Specification

13.060.70   Examination of biological properties of water



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ISO/TS 18220:2016 specifies an early-life stage procedure for determination of the toxic effects of chemicals and water samples on a cold-water brackish water copepod species under semi-static conditions. The biological test variables include survival and development of the early-life stages. The exposure starts with newly hatched (<24 h) nauplii (larvae) and is continued until emergence of (c. 50 %) copepodites (juveniles) in the control.
The benthic living Nitocra complements the planktonic Acartia species in ISO 16778. These organisms represent different life-history strategies as Nitocra is egg-carrying, whereas Acartia is a broadcasting calanoid copepod and thus, different sensitivities of specific life stages. Nitocra is a fresh to brackish water species, which allows testing low salinity waters and is complementary to A. tonsa, which is of marine origin and poorly tolerates low salinities.

Life cycle


ISO/TS 18220:2016
90.93 Standard confirmed
Sep 16, 2019