ISO/TS 21569-2:2012

Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products

Publication date:   Sep 3, 2012

95.99 Withdrawal of Standard   Jul 5, 2021

General information

95.99 Withdrawal of Standard   Jul 5, 2021

ISO

ISO/TC 34/SC 16 Horizontal methods for molecular biomarker analysis

Technical Specification

67.050   General methods of tests and analysis for food products

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Scope

This method describes a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as "CDC Triffid"). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopalin synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.

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ISO/TS 21569-2:2012
95.99 Withdrawal of Standard
Jul 5, 2021

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ISO/TS 21569-2:2021