ISO/TS 15216-1:2013

Microbiology of food and animal feed — Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR — Part 1: Method for quantification ISO/TS 15216-1:2013

Publication date:   Mar 7, 2013

95.99 Withdrawal of Standard   Mar 9, 2017

General information

95.99 Withdrawal of Standard   Mar 9, 2017

ISO

ISO/TC 34/SC 9 Microbiology

Technical Specification

07.100.30   Food microbiology

Buying

Withdrawn

Language in which you want to receive the document.

Scope

ISO/TS 15216-1:2013 describes a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.

Life cycle

NOW

WITHDRAWN
ISO/TS 15216-1:2013
95.99 Withdrawal of Standard
Mar 9, 2017

REVISED BY

PUBLISHED
ISO 15216-1:2017