CEN/TS 17329-1:2019

Foodstuffs - General guidelines for the validation of qualitative real-time PCR methods - Part 1: Single-laboratory validation

Publication date:   Jul 15, 2019

General information

99.60 Withdrawal effective   Jun 23, 2021


CEN/TC 275 Food analysis - Horizontal methods

Technical Specification

67.050   General methods of tests and analysis for food products



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This document describes the performance characteristics and minimum performance criteria which should be taken into account when conducting a single-laboratory validation study for qualitative (binary) real-time polymerase chain reaction (PCR) methods applied for the detection of specific DNA sequences present in foods.
The protocol was developed for qualitative real-time PCR methods for the detection of DNA sequences derived from genetically modified foodstuffs. It is applicable also for single-laboratory validation of qualitative PCR methods used for analysis of other food materials, e.g. for species detection and identification.
The document does not cover the evaluation of the applicability and the practicability with respect to the specific scope of the PCR method.

Related legislation

Legislation related to this standard


Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules

Life cycle


CEN/TS 17329-1:2019
99.60 Withdrawal effective
Jun 23, 2021


CEN/TS 17329-1:2021


Adopted from BVL Design Guidelines for the singleLab IDENTICAL