EN 17504:2022

Animal feeding stuffs: Methods of sampling and analysis - Determination of gossypol in cotton seed and feeding stuff by LC-MS/MS

General information

60.60 Standard published   Mar 30, 2022


CEN/TC 327 Animal feeding stuffs - Methods of sampling and analysis

European Norm

65.120   Animal feeding stuffs


This document specifies a method for the determination of free gossypol, extractable by acidified acetonitrile/water, in cottonseed, cottonseed products and compound feeds by liquid chromatography with tandem mass spectrometry (LC-MS/MS).
The method described in this document has been successfully validated in the range of 69 mg/kg to 5 950 mg/kg by collaborative trial in the following matrices: cottonseed, cottonseed products (cake/meal, hulls) and compound feeds for bovine, porcine and poultry.
NOTE It is possible to reach quantification limits of approximatively 5 mg/kg in compound feeds. The method might be applicable at lower and at higher concentrations than the concentration range validated in the collaborative trial. However, this needs to be assessed by in-house validation.

Related legislation

Legislation related to this standard


Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules

Life cycle


EN 17504:2022
60.60 Standard published
Mar 30, 2022