prEN 17853

Animal feeding stuffs: Methods of sampling and analysis - Determination of intact glucosinolates in rapeseed by LC-MS/MS

General information

40.10 harmonizedStageCodeLabel.40.10   Mar 18, 2022

CEN

CEN/TC 327 Animal feeding stuffs - Methods of sampling and analysis

European Norm

65.120   Animal feeding stuffs

Scope

This document describes a method for the determination of individual intact glucosinolates in rapeseed
by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry
(MS/MS). Progoitrin, gluconapin, glucobrassicanapin, glucobrassicin, nasturtiin, neoglucobrassicin and
4-methoxyglucobrassicin are quantitatively determined. Other glucosinolates such as
4-hydroxyglucobrassicin, glucnapoliferin, glucoalysin, can only be qualitatively detected when
analytical standards become commercially available.
The method has been in-house validated for rapeseeds in the range 0.05-60 mmol/kg for individual
glucosinolates.

Related legislation

Legislation related to this standard

882/2004

Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules

Life cycle

NOW

IN_DEVELOPMENT
prEN 17853
40.10 harmonizedStageCodeLabel.40.10
Mar 18, 2022