EN ISO 9167:2019

Rapeseed and rapeseed meals - Determination of glucosinolates content - Method using high-performance liquid chromatography (ISO 9167:2019)

Publication date:   Oct 16, 2019

General information

60.60 Standard published   Jun 19, 2019

CEN

CEN/TC 307 Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis

European Norm

67.200.20   Oilseeds

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Scope

This document specifies a method for the determination of the individual glucosinolates content in rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution.
This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus and Brassica juncea) but is applicable to other plant materials, on the condition that the occurring glucosinolates previously identified are described in this document. On the contrary, the quantitative analysis of the concerned glucosinolate(s) is not carried out.
NOTE This method does not determine glucosinolates that are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed and rapeseed meal.
Annex A presents the results of the interlaboratory trials for the gradient elution HPLC method. Annex B presents how to check the titre of the prepared internal standard solution. Annex C presents how to prepare and test the purified sulfatase solution and how to check the desulphation step on the ion exchange column. Annex D presents the HPLC and column performance criteria qualification.
The analysis of glucosinolates content in rapeseed can also be done using an isocratic elution mode. This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers), as described in Annex E.

Life cycle

PREVIOUSLY

Replaces
EN ISO 9167-1:1995

Replaces
EN ISO 9167-1:1995/A1:2013

NOW

PUBLISHED
EN ISO 9167:2019
60.60 Standard published
Jun 19, 2019