EN ISO 21149:2017

Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria (ISO 21149:2017)

Publication date:   Aug 17, 2017

General information

60.60 Standard published   Jun 21, 2017


CEN/TC 392 Cosmetics

European Norm

07.100.99   Other standards related to microbiology | 71.100.70   Cosmetics. Toiletries



Language in which you want to receive the document.


ISO 21149:2017 gives general guidelines for enumeration and detection of aerobic mesophilic bacteria present in cosmetics
- by counting the colonies on agar medium after aerobic incubation, or
- by checking the absence of bacterial growth after enrichment.
Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.
If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography.
In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity, hydro-alcoholic products, extreme pH values, etc.

Life cycle


EN ISO 21149:2009


EN ISO 21149:2017
60.60 Standard published
Jun 21, 2017


Amended by
EN ISO 21149:2017/A1:2022