EN ISO 12966-2:2017

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2: Preparation of methyl esters of fatty acids (ISO 12966-2:2017)

Publication date:   Aug 17, 2017

General information

60.60 Standard published   Apr 26, 2017


CEN/TC 307 Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis

European Norm

67.200.10   Animal and vegetable fats and oils



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ISO 12966-2:2017 specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely:
a) a "rapid" transmethylation procedure under alkaline conditions;
b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions;
c) a BF3 transmethylation procedure;
d) an alternative procedure using acid-catalysed transmethylation of glycerides.
Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

Life cycle


EN ISO 12966-2:2011


EN ISO 12966-2:2017
60.60 Standard published
Apr 26, 2017