Main menu
60.60 Standard published Oct 23, 2019
CEN
CEN/TC 327 Animal feeding stuffs - Methods of sampling and analysis
European Norm
65.120 Animal feeding stuffs
Published
This document method is applicable for the determination of theobromine in compound feed by liquid chromatography with UV detection in the tested range of 27 to 307 mg/kg. This method has been validated using complementary compound feed for adult dogs and complementary compound feedstuff for horses. The actual working range may extend beyond the tested range. Alternative chromatography conditions using liquid chromatography tandem mass spectrometry (LC-MS/MS) are also provided for the validated range of 49 to 307 mg/kg. This method has also been shown to be fit for purpose for the determination of theobromine in baking chocolate by both HPLC-UV and LC-MS/MS.
Legislation related to this standard
Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
PUBLISHED
EN 17270:2019
60.60
Standard published
Oct 23, 2019