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CEN ISO/TS 15216-2:2013

Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2: Method for qualitative detection (ISO/TS 15216-2:2013, Corrected Version 2013-05-01) CEN ISO/TS 15216-2:2013

Publication date:   Jul 15, 2013

General information

99.60 Withdrawal effective   Sep 18, 2019

CEN

CEN/TC 275 Food analysis - Horizontal methods

Technical Specification

07.100.30   Food microbiology

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Scope

ISO/TS 15216-2:2013 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.

Life cycle

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CEN ISO/TS 15216-2:2013
99.60 Withdrawal effective
Sep 18, 2019

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EN ISO 15216-2:2019