EN 16158:2012

Animal feeding stuffs - Determination of semduramicin content - Liquid chromatographic method using a "tree" analytical approach EN 16158:2012

Publication date:   Jun 18, 2012

General information

90.60 Close of review   Mar 4, 2023

CEN

CEN/TC 327 Animal feeding stuffs - Methods of sampling and analysis

European Norm

65.120   Animal feeding stuffs

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Scope

This European standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the semduramicin content at authorized level in animal feeding stuffs [2], using mass spectrometry detection or post-column derivatization and (UV)-VIS detection (hereinafter UV detection). This method is applicable to poultry feed. The limit of quantitation is 1,0 mg/kg when mass spectrometry is used for detection and 3,0 mg/kg when the detection is performed by UV with post-column derivatization. Lower limits of quantitation are achievable but this is to be validated by the user.
The method allows the discrimination of semduramicin from monensin, salinomycin, narasin, maduramicin and lasalocid.

Related legislation

Legislation related to this standard

70/373/EC

Council Directive 70/373/EEC of 20 July 1970 on the introduction of Community methods of sampling and analysis for the official control of feeding-stuffs

Life cycle

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PUBLISHED
EN 16158:2012
90.60 Close of review
Mar 4, 2023