EN ISO 15216-1:2017

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017) EN ISO 15216-1:2017

Publication date:   Jul 12, 2017

General information

60.60 Standard published   Mar 29, 2017

CEN

CEN/TC 463 Microbiology of the food chain

European Norm

07.100.30   Food microbiology

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Scope

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

Related legislation

Legislation related to this standard

882/2004

Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules

Life cycle

PREVIOUSLY

WITHDRAWN
CEN ISO/TS 15216-1:2013

NOW

PUBLISHED
EN ISO 15216-1:2017
60.60 Standard published
Mar 29, 2017

CORRIGENDA / AMENDMENTS

PUBLISHED
EN ISO 15216-1:2017/A1:2021